Tools, Protocols

Established techniques in the laboratory include, in addition to standard techniques in molecular and cellular biology:

– Generation and phenotypic analysis of transgenic mice including

– Behavioural analysis of mice (memory and motor tests)

– Primary neuronal cultures, general cell culture

– Histology, immunohistochemistry, silver impregnation techniques

– C. elegans techniques including the ballistic bombardment to establish transgenic lines

– Transcriptomics mRNAs/miRNAs (SAGE, Affymetrix gene chips)

– Proteomics (HPLC methods plus iTRAQ quantitative proteomics)

– Molecular and biochemical techniques, enzymatic assays, ELISAs

– Active and passive immunisation, generation of monoclonal antibodies


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